Search results for " serotype"
Article
Gene Therapies Push Viral Vector Production
By now, a wide variety of AAV serotypes have been studied, adds Kai Touw, technical lead at Batavia Biosciences, a Netherlands-based contract development and manufacturing organization (CDMO). Some of…
Article
Balancing AAVs Versus LLVs
The prevailing understanding is that serotypes bind to different, or have differential affinities to an array of primary cell surface glycoprotein receptors and secondary receptors.
Pharmace…
Article
A Look at the Affinity Chromatography Landscape
Baker points to ligands for AAV vector purification with both broad and specific serotype selectivities. “These developments have made the purification of certain vectors much more efficient and consi…
Article
How to optimize AAV capsid separation
In this free, interactive course you’ll learn how to tackle one of the key challenges in Adeno-associated virus (AAV) manufacturing–separating full and empty capsids in the polishing step. You’ll …
Article
Single-Use Technologies Prove Effective for Viral Vector Process Development
Even so, Sanderson observes that adherent bioreactors have a process advantage over suspension bioreactors for intracellular products such as those based on the AAV2 serotype, in which the attachment …
Article
Alternative solutions to separate AAV full and empty capsids using anion exchange
Here we present high-performance anion exchange (AEX) chromatography separation for several AAV serotypes using step-elution mode. Optimized protocols and conditions for both resin and membrane format…
Article
A Closer Look at Affinity Ligands
A new generation of vector serotype specific and non-specific affinity ligand chromatography resins are proving successful in capturing LV and AAV particles from cell-free media and promise the potent…
Article
A deep dive into optimizing AAV capture and polishing to maximize both yield and purity
Join this webinar to:
• Learn how to optimize affinity capture
• Discover critical parameters that enhance full and empty capsid separation
• See polishing data for several rAAV serotypes
…
Article
How to develop a scalable rAAV process
We produce and purify several serotypes, optimizing each step along the way. The key for a successful process are high overall yields of full capsids with empty capsid reduction and efficient impurity…
Article
From cells to purified capsids: How to develop a scalable rAAV process
We produce and purify several serotypes, optimizing each step along the way. The key for a successful process is high overall yields of full capsids with empty capsid reduction and efficient impurity …